ABSTRACT
Staphylococcus aureus is a Gram-possitive coccus having ability to produce
enterotoxin. It is reponsible for one of the most common types of food poisoning. In
testing food and finding the reason causing food poisoning nowadays, we have mainly
testsed the present of S. aureus, not enterotoxin which is the primary caussative agent
of Staphylococcal food poisoning. Therefore, in oder to get information of producing
enterotoxin, we carry on examining S. aureus growth and its ability to produce
enterotoxin in culture medium that contributes practically in testing food, especially in
Staphylococcal food poisoning outbreaks. S. aureus strains are cultured in TSGM and
BHI medium; then we examine their growth and test SE by ELISA method with
TECRA kit after 16, 24, 48 and 72 hours.
The results we find:
Among 36 strains surveyed, there are ten ones having ability to produce
SE (27,8%). In these ten strains, the ones from clinical samples have the highest rate
(50%).
S. aureus growth and SE amount are not correlative. SE amount
increases with time. After 16 hours culture, on TSGM, as the cell concentation reaches
7,86 log10 cfu/ml, OD ELISA value is 1,464; on BHI, as the cell concentation reaches
8,13 log10 cfu/ml OD, E
TSGM and BHI medium are the same influence for S. aureus growth
and ability to produce enterotoxin.
These S. aureus strains producce SEA, SEB, SEC. Among them, the rate
of SEA is 80%, of SEB is 10% and of SEC is 10%.